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Secondary structure of Streptococcus downei GTF-I glucansucrase

Lookup NU author(s): Dr Vincent Monchois, Professor Jeremy Lakey, Emeritus Professor Roy Russell

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Abstract

Multiple sequence alignment and structure prediction of glucansucrases produced by oral streptococci and Leuconostoc mesenteroides showed that all have common structural features, with three major domains. There is no conservation of primary sequence or structure in the N-terminal variable region. Sequence-based structure prediction combined with circular dichroism spectrum analysis of purified truncated forms of Streptococcus downei GTF-I revealed that the core catalytic region has a defined structure consistent with the proposed (α/β)8-barrel structure. The C-terminal domain is a mixed structure with significant amounts of β-sheet and random-coil. This information contributes to the development of our understanding of structure-function relationships in glucansucrases. Copyright (C) 1999 Federation of European Microbiological Societies.


Publication metadata

Author(s): Monchois V, Lakey JH, Russell RRB

Publication type: Article

Publication status: Published

Journal: FEMS Microbiology Letters

Year: 1999

Volume: 177

Issue: 2

Pages: 243-248

Print publication date: 15/08/1999

ISSN (print): 0378-1097

ISSN (electronic): 1574-6968

Publisher: Wiley-Blackwell Publishing Ltd.

URL: http://dx.doi.org/10.1016/S0378-1097(99)00317-1

DOI: 10.1016/S0378-1097(99)00317-1

PubMed id: 10474191


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