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TGF-β expression in renal transplant biopsies - A comparative study between cyclosporin-A and tacrolimus

Lookup NU author(s): Dr Helen Robertson, Professor John Kirby, David Talbot

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Abstract

Background. Chronic rejection is a major cause of graft dysfunction after kidney transplantation. This fibroproliferative disease may be promoted by overproduction of transforming growth factor beta (TGF-β). Previous studies have suggested that CsA might increase production of this growth factor. The current study was designed to measure the expression of TGF-βb in renal transplant biopsy specimens from patients undergoing immunosuppressive therapy with either CsA or tacrolimus (FK506). Method. Paraffin-embedded renal biopsy specimens were sectioned, dewaxed, and incubated with primary antibody against TGF-βb1 latency-associated protein and active TGF-βb1. After washing, the sections were treated with secondary antibody conjugated with FITC. In each case, the sections were assessed by semiquantitative scanning laser confocal microscopic method. Results. There was no significant difference in latent TGF-βb expression between biopsy specimens from patients receiving CsA and patients receiving FK506. However, biopsy specimens from patients receiving CsA expressed significantly more active TGF-βb1 than biopsy specimens from patients receiving FK506 (P<0.0001, Mann-Whitney test). Discussion. The increased level of active TGF- β1 expression in renal biopsy specimens of patients receiving CsA may indicate a mechanism of chronic rejection. However, these biopsies were performed to assess deranged renal function; therefore, the specimens may reflect events rather than differences in medication.


Publication metadata

Author(s): Kirby JA; Robertson H; Talbot D; Mohamed MAS; Booth TA; Balupuri S

Publication type: Article

Publication status: Published

Journal: Transplantation

Year: 2000

Volume: 69

Issue: 5

Pages: 1002-1005

ISSN (print): 0041-1337

ISSN (electronic): 1534-6080

Publisher: Lippincott Williams & Wilkins

URL: http://dx.doi.org/10.1097/00007890-200003150-00059

DOI: 10.1097/00007890-200003150-00059

PubMed id: 10755567


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