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The effect of NAT2 genotype and gender on the metabolism of caffeine in nonsmoking subjects

Lookup NU author(s): Dr Mark Welfare, Professor Margaret Bassendine, Professor Ann Daly

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Abstract

Aims. To establish whether gender or N-acetyltransferase 2 (NAT2) genotype influence the urinary 17 U + 17X/137X ratio after dosing with caffeine. Methods. Ninety-two nonsmoking individuals underwent caffeine phenotyping. NAT2 genotype was determined by the polymerase chain reaction followed by a restriction digest (PCR-RFLP). Results. The median ratio for urinary 17 U + 17X/137X was 6.7 (range 1.45-18.65). 55% of subjects were slow acetylators. Gender did not affect the metabolic ratio or NAT2 genotype. Mean 17 U + 17X/137X ratio differed between fast (6.75) and slow (8.69) acetylators (95% CI for the difference, 0.32-3.56). Conclusions. The findings are further evidence that the 17 U + 17X/137X urinary ratio is not a robust measure of CYP1A2 activity. A possible mechanism by which the ratio might be influenced by NAT2 genotype is suggested.


Publication metadata

Author(s): Daly AK; Bassendine MF; Welfare MR

Publication type: Article

Publication status: Published

Journal: British Journal of Clinical Pharmacology

Year: 2000

Volume: 49

Issue: 3

Pages: 240-243

ISSN (print): 0306-5251

ISSN (electronic): 1365-2125

Publisher: Wiley-Blackwell Publishing Ltd.

URL: http://dx.doi.org/10.1046/j.1365-2125.2000.00130.x

DOI: 10.1046/j.1365-2125.2000.00130.x

PubMed id: 10718779


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