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Absence of expression from RNA internalised into electroporated mammalian mitochondria

Lookup NU author(s): Dr Richard Temperley, Professor Zofia Chrzanowska-Lightowlers, Professor Robert Lightowlers

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Abstract

Transfection of mammalian mitochondria has proved to be notoriously difficult. Whilst there have been sporadic reports of import of foreign nucleic acids into isolated organelles, these imported nucleic acids have never been demonstrated to be functional. Inability to manipulate mitochondrial gene expression has hampered our understanding of RNA processing, maturation and translation in mitochondria. In an attempt to establish a model system for mt-RNA expression, we have electroporated rat liver mitochondria and mitoplasts in the presence of various RNA constructs built around the mitochondrial reporter gene mt-luciferase. Following electroporation, a fraction of the RNA was shown to be stably maintained, mitochondria remained coupled for oxidative phosphorylation and intramitochondrial protein synthesis was unaffected. In no case, however, was this RNA translated.


Publication metadata

Author(s): McGregor A, Temperley R, Chrzanowska-Lightowlers Z, Lightowlers NR

Publication type: Article

Publication status: Published

Journal: Molecular Genetics and Genomics

Year: 2001

Volume: 265

Issue: 4

Pages: 721-729

Print publication date: 01/06/2001

ISSN (print): 1617-4615

ISSN (electronic): 1617-4623

Publisher: Springer

URL: http://dx.doi.org/10.1007/s004380100469

DOI: 10.1007/s004380100469

PubMed id: 11459193


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