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RXRβ isoforms in neuroblastoma cells and evidence for a novel 3′-end transcript

Lookup NU author(s): Dr Birju Rana, Professor Andrew Pearson, Dr Chris Redfern

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Abstract

RXRβ is predominantly involved in retinoid responses in neuroblastoma cells, in particular the N-type SH SY 5Y cells and the S-type SH S EP cells, both derivatives of a mixed phenotype neuroblastoma cell line. The aim of this study was to identify RXRβ isoforms expressed in neuroblastoma cells and to characterise a putative novel RXRβ transcript. RXRβ1 and RXRβ2 were expressed in these neuroblastoma cells. An isoform with an insertion into the ligand binding domain, RXRβSLSR (referred to in previous studies as RXRβ3), was expressed at a similar level to RXRβ. A novel RXRβ transcript was identified by RNase protection assays and was at least as abundant as the expected RXRβ transcript and expressed in other cell types. Evidence suggests that this novel transcript was transcribed from an internal promoter between exons 5 and 6, contained a retained intron (intron 6) and was alternatively spliced with and without the SLSR insertion. These data show that the pattern of RXRβ expression is complex. The relative abundance of the novel RXRβ transcript suggests that it may be an important aspect of RXRβ function or regulation in a range of cell types. © 2001 Federation of European Biochemical Societies. Published by Elsevier Science B.V. All rights reserved.


Publication metadata

Author(s): Rana, B., Pearson, A.D.J., Redfern, C.P.F.

Publication type: Article

Publication status: Published

Journal: FEBS Letters

Year: 2001

Volume: 506

Issue: 1

Pages: 39-44

Print publication date: 28/09/2001

ISSN (print): 0014-5793

ISSN (electronic): 1873-3468

URL: http://dx.doi.org/10.1016/S0014-5793(01)02882-4

DOI: 10.1016/S0014-5793(01)02882-4

PubMed id: 11591367


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