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Targeting polymerised liposome vaccine carriers to intestinal M cells

Lookup NU author(s): Dr Helen Blair, Professor Barry Hirst

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Abstract

Due to their transcytotic capability, intestinal M cells may represent an efficient potential route for oral vaccine delivery. We previously demonstrated that the lectin Ulex europaeus agglutinin 1 (UEA1, specific for α-l-fucose residues) selectively binds to mouse Peyer's patch M cells and targets 0.5 μm polystyrene microparticles to these cells. Using a gut loop model we now demonstrate that covalently-membrane-bound UEA1 similarly targets polymerised liposomes (Orasomes™, approximately 200 nm diameter), potential biocompatable oral vaccine delivery vehicles, to mouse M cells. Targeting was inhibited by α-l-fucose while the co-entrapped adjuvant, monophosphoryl Lipid A (MPL®), failed to exert any detrimental effect on UEA1-mediated M cell targeting. Lectin-mediated M cell targeting may thus permit the efficacy of mucosal vaccines to be enhanced if cellular relationship between particle binding and immune outcome can be established. © 2001 Elsevier Science Ltd. All rights reserved.


Publication metadata

Author(s): Hirst BH; Blair H; Ann Clark M; Liang L; Brey RN; Brayden D

Publication type: Article

Publication status: Published

Journal: Vaccine

Year: 2001

Volume: 20

Issue: 1-2

Pages: 208-217

ISSN (print): 0264-410X

ISSN (electronic): 1873-2518

Publisher: Elsevier

URL: http://dx.doi.org/10.1016/S0264-410X(01)00258-4

DOI: 10.1016/S0264-410X(01)00258-4

PubMed id: 11567766


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