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Cultured glial cells are resistant to the effects of motor neurone disease-associated SOD1 mutations

Lookup NU author(s): Dr Rebecca Williams, Dr Mark Cookson, Dr Anne Fray, Dr Philip Manning, Fiona Menzies, Professor Pamela Shaw

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Abstract

Free radical damage has been implicated in the pathophysiology of motor neurone disease (MND); mutations have been identified in the gene encoding Cu/Zn superoxide dismutase (SOD1). There is evidence that glial cell dysfunction may contribute to motor neurone injury, but the exact role of glial cells in MND has yet to be established. The aim of this study was to determine whether expression of mutant SOD1 affects the response of glia to oxidative stress. Stable C6 glioma cells expressing mutant SOD1 and cortical astrocyte cultures from G93A-SOD1 transgenic mice were exposed to: xanthine/xanthine oxidase; hydrogen peroxide; A23187 and 3-morpholinosydonimine. Cell viability was measured using the 3-(4,5-dimethyl-thiazol-2-yl)-2,5-diphenyltetrazolium bromide assay. Neither C6 glioma cells nor cortical astrocytes expressing mutant SOD1 were more susceptible to any of the free radical generating systems compared to control cells. These results suggest that astrocytes are resistant to the toxic effects of mutant SOD1 widely reported for neuronal cells. © 2001 Elsevier Science Ireland Ltd.


Publication metadata

Author(s): Williams RE, Cookson MR, Fray AE, Manning PM, Menzies FM, Figlewicz DA, Shaw PJ

Publication type: Article

Publication status: Published

Journal: Neuroscience Letters

Year: 2001

Volume: 302

Issue: 2-3

Pages: 146-150

ISSN (print): 0304-3940

ISSN (electronic): 1872-7972

Publisher: Elsevier Ireland Ltd

URL: http://dx.doi.org/10.1016/S0304-3940(01)01686-X

DOI: 10.1016/S0304-3940(01)01686-X

PubMed id: 11290408


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