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Differentiation between Mycobacterium farcinogenes and Mycobacterium senegalense strains based on 16S-23S ribosomal DNA internal transcribed spacer sequences

Lookup NU author(s): Dr Mohamed Hamid, Professor Michael Goodfellow

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Abstract

16S ribosomal DNA (rDNA) and 16S-23S internal transcribed spacer rDNA sequence analyses were performed on Mycobacterium farcinogenes and M. senegalense strains and 26 strains of other rapidly growing mycobacteria to investigate the phylogenetic structure of bovine farcy mycobacteria within the M. fortuitum complex. M. farcinogenes and M. senegalense were indistinguishable in their 5′-end 16S rDNA but showed both considerable interspecies spacer sequence divergence and a high level of intraspecies sequence stability. A rapid detection assay using PCR and hybridization with species specific probes was developed. The assay was specific among 46 species other than M. farcinogenes and M. senegalense and correctly identified all M. farcinogenes and M. senegalense strains. PCR- and 16S-23S rDNA sequence-based detection will be a valuable approach for diagnosis of the causal agents of African bovine farcy in cattle.

Publication metadata

Author(s): Goodfellow M; Hamid ME; Roth A; Landt O; Kroppenstedt RM; Mauch H

Publication type: Article

Publication status: Published

Journal: Journal of Clinical Microbiology

Year: 2002

Volume: 40

Issue: 2

Pages: 707-711

ISSN (print): 0095-1137

ISSN (electronic): 1098-660X

Publisher: American Society for Microbiology

URL: http://dx.doi.org/10.1128/JCM.40.2.707-711.2002

DOI: 10.1128/JCM.40.2.707-711.2002

PubMed id: 11826003

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