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Paramagnetic 1H NMR spectrum of nickel(II) pseudoazurin: Investigation of the active site structure and the acid and alkaline transitions

Lookup NU author(s): Professor Christopher Dennison, Dr Katsuko Sato

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Abstract

The paramagnetic 1H NMR spectrum of Ni(II) pseudoazurin [(PA)NiII] possesses a number of resonances exhibiting sizable Fermi-contact shifts. These have been assigned to protons associated with the four ligating amino acids, His40, Cys78, His81, and Met86. The shifts experienced by the CγH protons of the axial Met86 ligand are unprecedented compared to other Ni(II)- and Co(II)-substituted cupredoxins (the Cγ1H signal is found at 432.5 ppm at 25 °C). The large shift of protons of the axial Met86 ligand highlights a strong Ni(II)-S(Met) interaction in (PA)NiII. The paramagnetic 1H NMR spectrum of (PA)NiII is altered by decreasing and increasing the pH value from 8.0. At acidic pH a number of the hyperfine-shifted resonances undergo limited changes in their chemical shift values. This effect is assigned to the surface His6 residue whose protonation results in a structural modification of the active site. Increasing the pH value from 8.0 has a more significant effect on the paramagnetic 1H NMR spectrum of (PA)NiII, and the alkaline transition can now be assigned to two surface lysine residues close to the active site of the protein. The effect of altering pH on the 1H NMR spectrum of Ni(II) pseudoazurin is smaller than that previously observed in the Cu(II) protein indicating more limited structural rearrangements at the non-native metal site.


Publication metadata

Author(s): Sato K; Dennison C

Publication type: Article

Publication status: Published

Journal: Inorganic Chemistry

Year: 2002

Volume: 41

Issue: 25

Pages: 6662-6672

ISSN (print): 0020-1669

ISSN (electronic): 1520-4995

Publisher: American Chemical Society

URL: http://dx.doi.org/10.1021/ic020303p

DOI: 10.1021/ic020303p


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