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Large-scale production and purification of recombinant Galanthus nivalis agglutinin (GNA) expressed in the methylotrophic yeast Pichia pastoris

Lookup NU author(s): Romaan Jan Maria Raemaekers, Professor Angharad MR Gatehouse

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Abstract

The gene coding for agglutinin from Galanthus nivalis (GNA) was expressed in, and secreted by, the methylotrophic yeast, Pichia pastoris. Transformants of P. pastoris were selected and a process to produce and purify gram quantities of recombinant GNA was developed. GNA was secreted at approximately 80 mg l-1 at the 200 1 scale and was purified to 95% homogeneity using hydrophobic interaction chromatography. The recombinant protein was similar to the protein synthesised in plant with respect to structure and biological activity.


Publication metadata

Author(s): Baumgartner P, Harper K, Raemaekers RJM, Durieux A, Gatehouse AMR, Davies HV, Taylor MA

Publication type: Article

Publication status: Published

Journal: Biotechnology Letters

Year: 2003

Volume: 25

Issue: 15

Pages: 1281-1285

Print publication date: 01/08/2003

ISSN (print): 0141-5492

ISSN (electronic): 1573-6776

Publisher: Springer

URL: http://dx.doi.org/10.1023/A:1025007901322

DOI: 10.1023/A:1025007901322

PubMed id: 14514082


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