Toggle Main Menu Toggle Search

Open Access padlockePrints

Large-scale production and purification of recombinant Galanthus nivalis agglutinin (GNA) expressed in the methylotrophic yeast Pichia pastoris

Lookup NU author(s): Romaan Jan Maria Raemaekers, Professor Angharad MR GatehouseORCiD

Downloads

Full text for this publication is not currently held within this repository. Alternative links are provided below where available.


Abstract

The gene coding for agglutinin from Galanthus nivalis (GNA) was expressed in, and secreted by, the methylotrophic yeast, Pichia pastoris. Transformants of P. pastoris were selected and a process to produce and purify gram quantities of recombinant GNA was developed. GNA was secreted at approximately 80 mg l-1 at the 200 1 scale and was purified to 95% homogeneity using hydrophobic interaction chromatography. The recombinant protein was similar to the protein synthesised in plant with respect to structure and biological activity.


Publication metadata

Author(s): Baumgartner P, Harper K, Raemaekers RJM, Durieux A, Gatehouse AMR, Davies HV, Taylor MA

Publication type: Article

Publication status: Published

Journal: Biotechnology Letters

Year: 2003

Volume: 25

Issue: 15

Pages: 1281-1285

Print publication date: 01/08/2003

ISSN (print): 0141-5492

ISSN (electronic): 1573-6776

Publisher: Springer

URL: http://dx.doi.org/10.1023/A:1025007901322

DOI: 10.1023/A:1025007901322

PubMed id: 14514082


Altmetrics

Altmetrics provided by Altmetric


Share