Lookup NU author(s): Romaan Jan Maria Raemaekers,
Professor Angharad MR Gatehouse
Full text for this publication is not currently held within this repository. Alternative links are provided below where available.
The gene coding for agglutinin from Galanthus nivalis (GNA) was expressed in, and secreted by, the methylotrophic yeast, Pichia pastoris. Transformants of P. pastoris were selected and a process to produce and purify gram quantities of recombinant GNA was developed. GNA was secreted at approximately 80 mg l-1 at the 200 1 scale and was purified to 95% homogeneity using hydrophobic interaction chromatography. The recombinant protein was similar to the protein synthesised in plant with respect to structure and biological activity.
Author(s): Baumgartner P, Harper K, Raemaekers RJM, Durieux A, Gatehouse AMR, Davies HV, Taylor MA
Publication type: Article
Publication status: Published
Journal: Biotechnology Letters
Print publication date: 01/08/2003
ISSN (print): 0141-5492
ISSN (electronic): 1573-6776
PubMed id: 14514082
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