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The parsley plastocyanin-turnip cytochrome f complex: A structurally distorted but kinetically functional acidic patch

Lookup NU author(s): Dr David Hunter, Dr Katsuko Sato, Emeritus Professor William McFarlane, Professor Christopher Dennison

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Abstract

In general, inter-protein electron transfer proceeds via the formation of transient complexes. The initial stage of the interaction between plastocyanin (PCu) and cytochrome f (cyt f) from plants is mediated by complementary electrostatics. Given the diffuse nature of its acidic patch, parsley PCu is an atypical example of a plant PCu. The interaction of this PCu with turnip cyt f was investigated by stopped-flow kinetics, NMR spectroscopy and protein-docking simulations. We show that, despite the altered acidic patch, parsley PCu is as efficient as spinach PCu in accepting electrons from cyt f, over the physiological range of ionic strength. At high ionic strength, the rate constant for the reaction of cyt f with parsley PCu is twice that of the spinach protein. This difference in reactivity is attributed to variations in the hydrophobic patch of parsley PCu. The results of NMR studies and protein-docking simulations indicate that parsley PCu and its spinach analogue adopt different orientations in their complexes with cyt f.


Publication metadata

Author(s): Crowley PB, Hunter DM, Sato K, McFarlane W, Dennison C

Publication type: Article

Publication status: Published

Journal: Biochemical Journal

Year: 2004

Volume: 378

Issue: 1

Pages: 45-51

ISSN (print): 0264-6021

ISSN (electronic): 1470-8728

Publisher: Portland Press Ltd

URL: http://dx.doi.org/10.1042/BJ20031423

DOI: 10.1042/BJ20031423

PubMed id: 14585099


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