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Post-transcriptional regulation of the Bacillus subtilis pst operon encoding a phosphate-specific ABC transporter

Lookup NU author(s): Dr Nicholas Allenby, Nicola O'Connor, Dr Zoltan Pragai, Dr Noel Carter, Professor Anil Wipat, Emeritus Professor Alan Ward, Professor Colin Harwood

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Abstract

During phosphate starvation, Bacillus subtilis regulates genes in the PhoP regulon to reduce the cell's requirement for this essential substrate and to facilitate the recovery of inorganic phosphate from organic sources such as teichoic and nucleic acids. Among the proteins that are highly induced under these conditions is PstS, the phosphate-binding lipoprotein component of a high-affinity ABC-type phosphate transporter. PstS is encoded by the first gene in the pst operon, the other four members of which encode the integral membrane and cytoplasmic components of the transporter. The transcription of the pst operon was analysed using a combination of methods, including transcriptional reporter gene technology, Northern blotting and DNA arrays. It is shown that the primary transcript of the pst operon is processed differentially to maintain higher concentrations of PstS relative to other components of the transporter. The comparative studies have revealed limitations in the use of reporter gene technology for analysing the transcription of operons in which the messenger RNA transcript is differentially processed. © 2004 SGM.


Publication metadata

Author(s): Allenby NEE, O'Connor N, Pragai Z, Carter NM, Miethke M, Engelmann S, Hecker M, Wipat A, Ward AC, Harwood CR

Publication type: Article

Publication status: Published

Journal: Microbiology

Year: 2004

Volume: 150

Issue: 8

Pages: 2619-2628

Print publication date: 01/08/2004

ISSN (print): 1350-0872

ISSN (electronic): 1465-2080

Publisher: Society for General Microbiology

URL: http://dx.doi.org/10.1099/mic.0.27126-0

DOI: 10.1099/mic.0.27126-0

PubMed id: 15289558


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