Toggle Main Menu Toggle Search

Open Access padlockePrints

Calcium phosphate and calcium oxalate crystal handling is dependent upon CLC-5 expression in mouse collecting duct cells

Lookup NU author(s): Professor John Sayer, Dr Georgina Carr, Professor Nicholas Simmons

Downloads

Full text for this publication is not currently held within this repository. Alternative links are provided below where available.


Abstract

Defects in an intracellular chloride channel CLC-5 cause Dent's disease, an inherited kidney stone disorder. Using a collecting duct model, mIMCD-3 cells, we show expression of dimeric mCLC-5. Transient transfection of antisense CLC-5 reduces CLC-5 protein expression. Binding of both calcium phosphate (hydroxyapatite) and calcium oxalate monohydrate (COM) crystals overlaid onto mIMCD-3 cultures was affected by altered CLC-5 expression. Calcium phosphate crystal agglomerations (>10 μm) were minimal in control (9%) and sense (13%) CLC-5-transfected cells, compared to 66% of antisense CLC-5-transfected cells (P<0.001). Small calcium phosphate crystals (<10 μm) were found associated with 45% of sense CLC-5-treated cells, of which the majority (11/14 cells) appeared to be internalised within the cell. Calcium oxalate agglomerations (>10 μm) were also largely absent for controls or sense mCLC-5 transfectants (11% and 9% of cells, respectively) with COM crystal agglomerates predominating in antisense CLC-5 transfectants (66%, P<0.0001). We conclude that collecting duct cells with reduced CLC-5 expression lead to a tendency to form calcium crystal agglomeration, which may help explain the nephrocalcinosis and nephrolithiasis seen in Dent's disease. © 2004 Elsevier B.V. All rights reserved.


Publication metadata

Author(s): Sayer JA, Carr G, Simmons NL

Publication type: Article

Publication status: Published

Journal: Biochimica et Biophysica Acta (BBA) - Molecular Basis of Disease

Year: 2004

Volume: 1689

Issue: 1

Pages: 83-90

ISSN (print): 0925-4439

ISSN (electronic): 0006-3002

Publisher: Elsevier BV

URL: http://dx.doi.org/10.1016/j.bbadis.2004.02.007

DOI: 10.1016/j.bbadis.2004.02.007

PubMed id: 15158917


Altmetrics

Altmetrics provided by Altmetric


Actions

Find at Newcastle University icon    Link to this publication


Share