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Lookup NU author(s): Dr Thomas Ressiniotis,
Dr Mark Birch,
Professor Patrick Chinnery
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Objective: To test the hypothesis that genetic polymorphisms of the apolipoprotein E (APOE) gene are associated with primary open-angle glaucoma (POAG), based on the association between neurodegenerative diseases and the APOE genotype. Methods: Genomic DNA was examined from an unrelated cohort of 137 POAG patients and 75 control subjects from the ophthalmology department of the Royal Victoria Infirmary. The APOE allele frequency (ε2, ε3, and ε4 alleles) was studied by polymerase chain reaction amplification of the related locus (19q13.2), enzymatic digestion of the products, gel electrophoresis, and imaging under UV illumination. For statistical analysis, we used a logistic regression model that included intraocular pressure as a continuous variable to study the possible correlation between POAG and APOE allele frequency. Results: Logistic regression analysis showed no statistically significant association between the frequency of the APOE allele and POAG for the population studied, irrespective of the IOP (ε2 odds ratio, 0.82; 95% confidence interval, 0.12-5.79 [P=.84]; ε3 odds ratio, 0.39; 95% confidence interval, 0.10-1.49 [P=.17]; and ε4 odds ratio, 3.84; 95% confidence interval, 0.80-18.49 [P=.091). Conclusion: In our cohort, the APOE genotype does not constitute a risk factor for developing POAG, even in patients with normal-tension glaucoma. Clinical Relevance: Apolipoprotein E polymorphisms do not appear to be contributory to POAG.
Author(s): Ressiniotis T, Griffiths PG, Birch M, Keers S, Chinnery PF
Publication type: Article
Publication status: Published
Journal: Archives of Ophthalmology
ISSN (print): 0003-9950
ISSN (electronic): 1538-3601
Publisher: American Medical Association
PubMed id: 14769603
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