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Accumulation of NaV1 mRNAs at differentiating postsynaptic sites in rat soleus muscles

Lookup NU author(s): Mark Stocksley, Suad Awad, Professor Robert Lightowlers, Emeritus Professor Clarke Slater

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Abstract

Acetylcholine receptors (AChRs) and voltage-gated sodium channels (Na V1s) accumulate at different times in the development of the murine neuromuscular junction (NMJ). We used in situ hybridization to study the relationship of NaV1 mRNA accumulation to this difference. mRNAs encoding both muscle NaV1 isoforms, Nav1.4 and Na v1.5, were first concentrated at NMJs at birth, when the proteins start to accumulate. Within 4 weeks, Nav1.4 mRNA increased 5-fold at the NMJ while Nav1.5 mRNA became undetectable. NaV1 mRNA accumulation occurred even if the nerve was cut at birth. Like AChR mRNA, NaV1 mRNA accumulated at denervated synaptic sites on regenerating muscles and in response to ectopically expressed neural agrin. Clustering of NaV1 at the NMJ follows that of its mRNA while AChR clustering precedes its mRNA clustering by several days. This suggests that factors other than local mRNA upregulation determine the timing of clustering of these two important postsynaptic ion channels. © 2004 Elsevier Inc. All rights reserved.


Publication metadata

Author(s): Stocksley MA, Awad SS, Young C, Lightowlers RN, Brenner H-R, Slater CR

Publication type: Article

Publication status: Published

Journal: Molecular and Cellular Neuroscience

Year: 2005

Volume: 28

Issue: 4

Pages: 694-702

ISSN (print): 1044-7431

ISSN (electronic): 1095-9327

Publisher: Academic Press

URL: http://dx.doi.org/10.1016/j.mcn.2004.11.015

DOI: 10.1016/j.mcn.2004.11.015

PubMed id: 15797716


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