Toggle Main Menu Toggle Search

Open Access padlockePrints

Transcriptional profiling of Helicobacter pylori Fur- and iron-regulated gene expression

Lookup NU author(s): Dr Ulrike Mader, Dr Georg Homuth


Full text for this publication is not currently held within this repository. Alternative links are provided below where available.


Intracellular iron homeostasis is a necessity for almost all living organisms, since both iron restriction and iron overload can result in cell death. The ferric uptake regulator protein, Fur, controls iron homeostasis in most Gram-negative bacteria. In the human gastric pathogen Helicobacter pylori, Fur is thought to have acquired extra functions to compensate for the relative paucity of regulatory genes. To identify H. pylori genes regulated by iron and Fur, we used DNA array-based transcriptional profiling with RNA isolated from H. pylori 26695 wild-type and fur mutant cells grown in iron-restricted and iron-replete conditions. Sixteen genes encoding proteins involved in metal metabolism, nitrogen metabolism, motility, cell wall synthesis and cofactor synthesis displayed iron-dependent Fur-repressed expression. Conversely, 16 genes encoding proteins involved in iron storage, respiration, energy metabolism, chemotaxis, and oxygen scavenging displayed iron-induced Fur-dependent expression. Several Fur-regulated genes have been previously shown to be essential for acid resistance or gastric colonization in animal models, such as those encoding the hydrogenase and superoxide dismutase enzymes. Overall, there was a partial overlap between the sets of genes regulated by Fur and those previously identified as growth-phase, iron or acid regulated. Regulatory patterns were confirmed for five selected genes using Northern hybridizabon. In conclusion, H. pylori Fur is a versatile regulator involved in many pathways essential for gastric colonization. These findings further delineate the central role of Fur in regulating the unique capacity of H. pylori to colonize the human stomach. © 2005 SGM.

Publication metadata

Author(s): Ernst FD, Bereswill S, Waidner B, Stoof J, Mader U, Kusters JG, Kuipers EJ, Kist M, van Vliet AHM, Homuth G

Publication type: Article

Publication status: Published

Journal: Microbiology

Year: 2005

Volume: 151

Issue: 2

Pages: 533-546

Print publication date: 01/02/2005

ISSN (print): 1350-0872

ISSN (electronic): 1465-2080

Publisher: Society for General Microbiology


DOI: 10.1099/mic.0.27404-0

PubMed id: 15699202


Altmetrics provided by Altmetric


Find at Newcastle University icon    Link to this publication