Toggle Main Menu Toggle Search

Open Access padlockePrints

Genome-wide transcriptional analysis of the phosphate starvation stimulon of Bacillus subtilis

Lookup NU author(s): Dr Nicholas Allenby, Nicola O'Connor, Dr Zoltan Pragai, Emeritus Professor Alan Ward, Professor Anil Wipat, Professor Colin Harwood

Downloads

Full text for this publication is not currently held within this repository. Alternative links are provided below where available.


Abstract

Bacillus subtilis responds to phosphate starvation stress by inducing the PhoP and SigB regulons. While the PhoP regulon provides a specific response to phosphate starvation stress, maximizing the acquisition of phosphate (P 1) from the environment and reducing the cellular requirement for this essential nutrient, the SigB regulon provides nonspecific resistance to stress by protecting essential cellular components, such as DNA and membranes. We have characterized the phosphate starvation stress response of B. subtilis at a genome-wide level using DNA macroarrays. A combination of outlier and cluster analyses identified putative new members of the PhoP regulon, namely, yfkN (2′,3′ cyclic nucleotide 2′-phosphodiesterase), yurI (RNase), yjdB (unknown), and vpr (extracellular serine protease). YurI is thought to be responsible for the nonspecific degradation of RNA, while the activity of YfkN on various nucleotide phosphates suggests that it could act on substrates liberated by YurI, which produces 3′ or 5′ phosphoribonucleotides. The putative new PhoP regulon members are either known or predicted to be secreted and are likely to be important for the recovery of inorganic phosphate from a variety of organic sources of phosphate in the environment. Copyright © 2005, American Society for Microbiology. All Rights Reserved.


Publication metadata

Author(s): Allenby NEE, O'Connor N, Pragai Z, Ward AC, Wipat A, Harwood CR

Publication type: Article

Publication status: Published

Journal: Journal of Bacteriology

Year: 2005

Volume: 187

Issue: 23

Pages: 8063-8080

Print publication date: 01/12/2005

ISSN (print): 0021-9193

ISSN (electronic): 1067-8832

Publisher: American Society for Microbiology

URL: http://dx.doi.org/10.1128/JB.187.23.8063-8080.2005

DOI: 10.1128/JB.187.23.8063-8080.2005

PubMed id: 16291680


Altmetrics

Altmetrics provided by Altmetric


Actions

Find at Newcastle University icon    Link to this publication


Share