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Combined system for the simultaneous optical and electrochemical monitoring of intra- and extracellular NO produced by glioblastoma cells

Lookup NU author(s): Dr James Henderson, Emeritus Professor Calum McNeil

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Abstract

A combined, optospectroscopic and electrochemical assay system for the simultaneous monitoring of intra- and extracellular production of biologically important species has been developed and assessed. The present model system evaluates intra- and extracellular nitric oxide produced by stimulated glioblastoma multiform cell line (A172). The production of endogenous NO was induced by phorbol-12-myristate-13-acetate and inhibited by Nω-nitro-L- arginine methyl ester. Intracellular production of NO was monitored via fluorescence image analysis using a 4,5-diaminofluorescein probe, while extracellular NO release was monitored via a chemically modified electrode, which was incorporated into an optically transparent cell chip. The results indicated that there was no mutual interference between the optical and electrochemical measurement systems. The response time of the combined optical/electrochemical system was found to be in the range of a few tens of seconds. © 2005 American Chemical Society.


Publication metadata

Author(s): Pereira-Rodrigues N, Zurgil N, Chang S-C, Henderson JR, Bedioui F, McNeil CJ, Deutsch M

Publication type: Article

Publication status: Published

Journal: Analytical Chemistry

Year: 2005

Volume: 77

Issue: 9

Pages: 2733-2738

Print publication date: 01/05/2005

ISSN (print): 0003-2700

ISSN (electronic): 1520-6882

Publisher: American Chemical Society

URL: http://dx.doi.org/10.1021/ac0483163

DOI: 10.1021/ac0483163


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