Lookup NU author(s): Dr Phillip Aldridge,
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The σ28 protein is a member of the bacterial σ70-family of transcription factors that directs RNA polymerase to flagellar late (class 3) promoters. The σ28 protein is regulated in response to flagellar assembly by the anti-σ28 factor FlgM. FlgM inhibits σ28-dependent transcription of genes whose products are needed late in assembly until the flagellar basal motor structure, the hook-basal body (HBB), is constructed. A second function for the σ28 transcription factor has been discovered: σ28 facilitates the secretion of FlgM through the HBB, acting as the FlgM Type III secretion chaperone. Transcription-specific mutants in σ28 were isolated that remained competent for FlgM-facilitated secretion separating the transcription and secretion-facilitation activities of σ28. Conversely, we also describe the isolation of mutants in σ28 that are specific for FlgM-facilitated secretion. The data demonstrate that σ28 is the Type III secretion chaperone for its own anti-sigma factor FlgM. Thus, a novel role for a σ70- family transcription factor is described. © 2006 by Cold Spring Harbor Laboratory Press.
Author(s): Aldridge PD, Karlinsey JE, Aldridge C, Birchall C, Thompson D, Yagasaki J, Hughes KT
Publication type: Article
Publication status: Published
Journal: Genes & Development
Print publication date: 15/08/2006
ISSN (print): 0890-9369
ISSN (electronic): 1549-5477
Publisher: Cold Spring Harbor Laboratory Press
PubMed id: 16912280
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