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Fat depot-specific characteristics are retained in strains derived from single human preadipocytes

Lookup NU author(s): Dr Carmen Martin-Ruiz, Professor Thomas von Zglinicki

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Abstract

Fat depots vary in size, function, and potential contribution to disease. Since fat tissue turns over throughout life, preadipocyte characteristics could contribute to this regional variation. To address whether preadipocytes from different depots are distinct, we produced preadipocyte strains from single abdominal subcutaneous, mesenteric, and omental human preadipocytes by stably expressing human telomere reverse transcriptase (hTERT). These strains could be subcultured repeatedly and retained capacity for differentiation, while primary preadipocyte adipogenesis and replication declined with subculturing. Primary omental preadipocytes, in which telomeres were longest, replicated more slowly than mesenteric or abdominal subcutaneous preadipocytes. Even after 40 population doublings, replication, abundance of the rapidly replicating preadipocyte subtype, and resistance to tumor necrosis factor {alpha}-induced apoptosis were highest in subcutaneous, intermediate in mesenteric, and lowest in omental hTERT-expressing strains, as in primary preadipocytes. Subcutaneous hTERT-expressing strains accumulated more lipid and expressed more adipocyte fatty acid-binding protein (aP2), peroxisome proliferator-activated receptor {gamma}2, and CCAAT/enhancer-binding protein {alpha} than omental cells, as in primary preadipocytes, while hTERT abundance was similar. Thus, despite dividing 40 population doublings, hTERT strains derived from single preadipocytes retained fat depot-specific cell dynamic characteristics, consistent with heritable processes contributing to regional variation in fat tissue function. © 2006 by the American Diabetes Association.


Publication metadata

Author(s): Tchkonia T, Giorgadze N, Pirtskhalava T, Thomou T, De Ponte M, Koo A, Forse RA, Chinnappan D, Martin-Ruiz C, Von Zglinicki T, Kirkland JL

Publication type: Article

Publication status: Published

Journal: Diabetes

Year: 2006

Volume: 55

Issue: 9

Pages: 2571-2578

ISSN (print): 0012-1797

ISSN (electronic): 1939-327X

Publisher: American Diabetes Association

URL: http://dx.doi.org/10.2337/db06-0540

DOI: 10.2337/db06-0540

PubMed id: 16936206


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