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Identification of a non-mammalian leptin-like gene: Characterization and expression in the tiger salamander (Ambystoma tigrinum)

Lookup NU author(s): Dr Timothy Boswell

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Abstract

Leptin is well established as a multifunctional cytokine in mammals. However, little is known about the evolution of the leptin gene in other vertebrates. A recently published set of ESTs from the tiger salamander (Ambystoma tigrinum) contains a sequence sharing 56% nucleotide sequence identity with the human leptin cDNA. To confirm that the EST is naturally expressed in the salamander, a 409 bp cDNA was amplified by RT-PCR of salamander testis and stomach mRNAs. The coding sequence of the cDNA is predicted to encode 169 amino acids, and the mature peptide to consist of 146 residues, as in mammals. Although the overall amino acid identity with mammalian leptins is only 29%, the salamander and mammalian peptides share common structural features. An intron was identified between coding exons providing evidence that the sequence is present in the salamander genome. Phylogenetic analysis showed a rate of molecular divergence consistent with the accepted view of vertebrate evolution. The pattern of tissue expression of the leptin-like cDNA differed between metamorphosed adult individuals of different sizes suggesting possible developmental regulation. Expression was most prominent in the skin and testis, but was also detected in tissues in which leptin mRNA is present in mammals, including the fat body, stomach, and muscle. The characterization of a salamander leptin-like gene provides a basis for understanding how the structure and functions of leptin have altered during the evolution of tetrapod vertebrates. © 2006 Elsevier Inc. All rights reserved.


Publication metadata

Author(s): Boswell T, Dunn IC, Wilson PW, Joseph N, Burt DW, Sharp PJ

Publication type: Article

Publication status: Published

Journal: General and Comparative Endocrinology

Year: 2006

Volume: 146

Issue: 2

Pages: 157-166

ISSN (print): 0016-6480

ISSN (electronic): 1095-6840

Publisher: Academic Press

URL: http://dx.doi.org/10.1016/j.ygcen.2005.08.001

DOI: 10.1016/j.ygcen.2005.08.001

PubMed id: 16480984


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