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Glucokinase regulatory protein is associated with mitochondria in hepatocytes

Lookup NU author(s): Dr Catherine Arden, Professor Loranne Agius

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Abstract

The association of glucokinase with liver mitochondria has been reported [Danial et al. (2003) BAD and glucokinase reside in a mitochondrial complex that integrates glycolysis and apoptosis. Nature 424, 952-956]. We confirmed association of glucokinase immunoreactivity with rat liver mitochondria using Percoll gradient centrifugation and demonstrated its association with the 68 kDa regulatory protein (GKRP) but not with the binding protein phosphofructokinase- 2/fructose bisphosphatase-2. Substrates and glucagon induced adaptive changes in the mitochondrial glucokinase/GKRP ratio suggesting a regulatory role for GKRP. Combined with previous observations that GKRP overexpression partially inhibits glycolysis [de la Iglesia et al. (2000) The role of the regulatory protein of glucokinase in the glucose sensory mechanism of the hepatocyte. J. Biol. Chem. 275, 10597-10603] these findings suggest that there may be distinct glycolytic pools of glucokinase. © 2006 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.


Publication metadata

Author(s): Arden C, Baltrusch S, Agius L

Publication type: Article

Publication status: Published

Journal: FEBS Letters

Year: 2006

Volume: 580

Issue: 8

Pages: 2065-2070

Print publication date: 03/04/2006

ISSN (print): 0014-5793

ISSN (electronic): 1873-3468

Publisher: Elsevier

URL: http://dx.doi.org/10.1016/j.febslet.2006.03.009

DOI: 10.1016/j.febslet.2006.03.009

PubMed id: 16542652

Notes: Edited by Vladimir Skulachev


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