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Nitric oxide stimulates insulin gene transcription in pancreatic β-cells

Lookup NU author(s): Dr Susan Campbell, Dr Hannah Richardson, Dr Clive Butler

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Abstract

Recent studies have identified a positive role for nitric oxide (NO) in the regulation of pancreatic β-cell function. The aim of this study was to determine the effects of short-term exposure to NO on β-cell gene expression and the activity of the transcription factor PDX-1. NO stimulated the activity of the insulin gene promoter in Min6 β-cells and endogenous insulin mRNA levels in both Min6 and isolated islets of Langerhans. Addition of wortmannin prior to NO stimulation blocked the observed increases in insulin gene promoter activity. Although NO addition stimulated the phosphorylation of p38, inhibition by SB203580 did not block the effect of NO on the insulin gene promoter. NO addition also stimulated both the nuclear accumulation and the DNA binding activity of PDX-1. This study has shown that over 24 h, NO stimulates insulin gene expression, PI-3-kinase activity and the activity of the critical β-cell transcription factor PDX-1. © 2006 Elsevier Inc. All rights reserved.


Publication metadata

Author(s): Campbell SC, Richardson H, Ferris WF, Butler CS, Macfarlane WM

Publication type: Article

Publication status: Published

Journal: Biochemical and Biophysical Research Communications

Year: 2007

Volume: 353

Issue: 4

Pages: 1011-1016

ISSN (print): 0006-291X

ISSN (electronic): 1090-2104

Publisher: Academic Press

URL: http://dx.doi.org/10.1016/j.bbrc.2006.12.127

DOI: 10.1016/j.bbrc.2006.12.127

PubMed id: 17210120


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