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Loss of Sox9 function results in defective chondrocyte differentiation of mouse embryonic stem cells in vitro

Lookup NU author(s): Dr Ralf KistORCiD

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Abstract

The transcription factor Sox9 plays an important role during chondrogenesis. After early conditional inactivation of Sox9 in mesenchymal limb bud cells of mice, mesenchymal condensations as well as cartilage and bone are completely absent in the developing limbs. We analyzed chondrogenic differentiation of Sox9-/- mouse embryonic stem cells in vitro, using two clones with different targeted mutations. We found that the development of mature and hypertrophic chondrocytes is completely inhibited in the absence of Sox9 confirming that Sox9 is required for the formation of cartilage. In contrast, Sox9+/- mouse embryonic stem cells showed continous but reduced differentiation into mature chondrocytes. Interestingly, the formation of early chondrogenic condensations expressing characteristic marker genes such as scleraxis, Sox5 and Sox6 was not inhibited in the absence of Sox9 in vitro. Thus, we propose that the earliest step of chondrogenesis could be regulated by a non cell-autonomous function of Sox9. © UBC Press.

Publication metadata

Author(s): Hargus G, Kist R, Kramer J, Gerstel D, Neitz A, Scherer G, Rohwedel J

Publication type: Article

Publication status: Published

Journal: International Journal of Developmental Biology

Year: 2008

Volume: 52

Issue: 4

Pages: 323-332

Print publication date: 01/01/2008

ISSN (print): 0214-6282

ISSN (electronic): 1696-3547

Publisher: Universidad del Pais Vasco

URL: http://dx.doi.org/10.1387/ijdb.072490gh

DOI: 10.1387/ijdb.072490gh

PubMed id: 18415932

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