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Characterisation of micromonosporae from aquatic environments using molecular taxonomic methods

Lookup NU author(s): Dr Luis Maldonado, Dr James Stach, Emeritus Professor Alan Ward, Professor Alan Bull, Professor Michael Goodfellow

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Abstract

Large numbers of strains assigned to the genus Micromonospora on the basis of typical colonial and pigmentation features were isolated from diverse aquatic sediments using a standard selective isolation procedure. Two hundred and six isolates and eight representatives of the genus Micromonospora were assigned to 24 multimembered groups based on a numerical analysis of banding patterns generated using BOX and ERIC primers. Representatives of multimembered groups encompassing isolated micromonosporae were the subject of 16S rRNA gene sequencing analyses. Good congruence was found between the molecular fingerprinting and 16S rRNA sequence data indicating that the groups based upon the former are taxonomically meaningful. Nearly all of the isolates that were chosen for the 16S rRNA gene sequencing analyses showed that the ecosystems studied are a rich source of novel micromonosporae. These findings have implications for high throughput screening for novel micromonosporae as BOX and ERIC fingerprinting, which is rapid and reproducible, can be applied as a robust dereplication procedure to indicate which environmental isolates have been cultured previously. © Springer Science+Business Media B.V. 2008.


Publication metadata

Author(s): Maldonado LA, Stach JEM, Ward AC, Bull AT, Goodfellow M

Publication type: Article

Publication status: Published

Journal: Antonie van Leeuwenhoek

Year: 2008

Volume: 94

Issue: 2

Pages: 289-298

ISSN (print): 0003-6072

ISSN (electronic): 1572-9699

Publisher: Springer

URL: http://dx.doi.org/10.1007/s10482-008-9244-0

DOI: 10.1007/s10482-008-9244-0


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