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Lookup NU author(s): Dr Trevor Thomas,
Dr Ian West
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Aim:The aim of this study was, a) to investigate the effect of mild oxidative stress on glycolytic fux and glyceraldehyde-3-phosphate dehydrogenase (GAPDH) binding in erythrocytes from patients with autosomal dominant polycystic kidney disease (ADPKD), and b) to examine whether the modulation of GAPDH-binding to the red cell membrane leads to changes in glycolytic fux. Patients and methods: The rate of lactate production in intact erythrocytes and the GAPDH/actin ratio in erythrocyte ghost membranes were measured before and after treating cells with t-butyl hydroperoxide or N-ethylmaleimide (NEM) in 13 ADPKD patients and 12 controls. Results: t-bytyl hydro-peroxide had a signifcant effect on both lactate production and GAPDH/actin ratio in healthy subjects, but it had essentially no effect on ADPKD patients in which both parameters already resembled those of the peroxide-treated controls. NEM treatment after 300 sec had a very signifcant effect on both lactate production and GAPDH/actin ratio in both patient and control cells. However, after 10 sec the effect on GAPDH/actin ratio was only signifcant in the erythrocytes of ADPKD patients. In every experiment glycolytic lactate production correlated negatively with membrane-bound GAPDH/actin ratio. Conclusions: We conclude that glycolytic fux and GAPDH binding in erythrocytes from ADPKD patients respond abnormally to both a mild oxidative stress and brief exposure to NEM.
Author(s): Dioudis C, Grekas D, Thomas TH, West IC
Publication type: Article
Publication status: Published
Print publication date: 01/01/2008
ISSN (print): 1108-4189
Publisher: Hippokratio General Hospital of Thessaloniki