Lookup NU author(s): Dr Christophe Noel
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Background: We characterised 37 amoebae cultured from corneal scrapings, contact lenses or lens case solutions of patients with suspected Acanthamoeba keratitis. Methods: The isolates were identified by their morphology and by PCR targeting the Acanthamoeba nuclear small-subunit rRNA gene. Acanthamoeba isolates were genotyped by DNA sequence analysis. Results: The 37 isolates comprised 35 Acanthamoeba, one Hartmannella and one Vahlkampfia. Ten Acanthamoeba isolates were shown to be responsible for keratitis. Conclusion: Genotype T4 was the only Acanthamoeba genotype responsible for keratitis in this study, and represented 79% of non-pathogenic isolates.
Author(s): Yera H, Zamfir O, Bourcier T, Viscogliosi E, Noel C, Dupouy-Camet J, Chaumeil C
Publication type: Article
Publication status: Published
Journal: British Journal of Ophthalmology
ISSN (print): 0007-1161
ISSN (electronic): 1468-2079
Publisher: BMJ Group
PubMed id: 18586902
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