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Determination of bactericidal activity of antibacterial monomer MDPB by a viability staining method

Lookup NU author(s): Emeritus Professor Roy Russell, Professor John McCabe

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Abstract

In this study, the bactericidal activity of antibacterial monomer MDPB (12-methacryloyloxydodecylpyridinium bromide) against Streptococcus mutans was tested by a rapid method for monitoring viability. To S. mutans culture containing fluorescence staining solution that distinguishes live from dead cells, MDPB was added at a concentration of 250, 100, 50, or 10 μg/ml. Bacterial cells were observed by fluorescence microscopy and the percentage of dead cells was calculated. After 10, 20, or 30 minutes' contact with MDPB, the live/dead ratio was measured by fluorometry and viable counts (CFU) determined by the conventional plating method. Viability staining revealed that MDPB exhibited significant bactericidal effects at 50 μg/ml or greater (ANOVA, Fisher's PLSD test), and complete killing of the cells at 250 μg/ml of MDPB was demonstrated in conjunction with a plating method. The staining method thus provided a sensitive means to determine loss of viability, and indicated the strong killing effects of MDPB on S. mutans.

Publication metadata

Author(s): Imazato S, Ohmori K, Russell RRB, McCabe JF, Momoi Y, Maeda N

Publication type: Article

Publication status: Published

Journal: Dental Materials Journal

Year: 2008

Volume: 27

Issue: 1

Pages: 145-148

Print publication date: 01/01/2008

ISSN (print): 0287-4547

ISSN (electronic): 1881-1361

Publisher: The Japanese Society for Dental Materials and Devices

URL: http://dx.doi.org/10.4012/dmj.27.145

DOI: 10.4012/dmj.27.145

PubMed id: 18309624

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