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Superoxide-induced nitric oxide release from cultured glial cells

Lookup NU author(s): Dr Philip Manning, Dr Mark Cookson, Emeritus Professor Calum McNeil, Professor Pamela Shaw

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Abstract

Nitric oxide (NO) has been implicated as a potential contributor to neural cell death in a variety of neurological conditions. Cultured glial cells were exposed to extracellular superoxide generated by the action of xanthine oxidase on xanthine. In this experimental paradigm, both C6 glioma cells and primary astrocytes from rat cerebral cortex produced a rapid release of nitric oxide, measured using an NO specific electrode, in response to the applied superoxide stimulus. Application of a superoxide scavenger, or over-expression of Cu/Zn superoxide dismutase decreased the observed NO release. Authenticity of the NO signal was confirmed by the addition of the NO scavenger 2-(carboxyphenyl)-4,4,5,5-tetramethyllimidazoline-1-oxyl 3-oxide (carboxy-PTIO), which abolished the observed NO release without affecting simultaneously measured superoxide. Therefore, we suggest that glial cells may produce NO under free radical stimulation, which may be relevant to several neurological disorders where superoxide radicals are generated in the vicinity of glia. This would be predicted to result in the release of NO, which may exert toxic effects on neighbouring cells. (C) 2001 Elsevier Science B.V. All rights reserved.


Publication metadata

Author(s): Manning P, Cookson MR, McNeil CJ, Figlewicz D, Shaw PJ

Publication type: Article

Publication status: Published

Journal: Brain Research

Year: 2001

Volume: 911

Issue: 2

Pages: 203-210

ISSN (print): 0006-8993

ISSN (electronic): 1872-6240

Publisher: Elsevier BV

URL: http://dx.doi.org/10.1016/S0006-8993(01)02688-9

DOI: 10.1016/S0006-8993(01)02688-9


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