Lookup NU author(s): Dr Rebecca Smith,
Professor Colin Harwood,
Dr Michael Barer
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The possibility that nonculturable cells of a normally culturable bacterial pathogen may constitute a source or reservoir for infective disease was investigated. in multiple experiments and with careful attention to the statistical limitations of the assays used, Salmonella enterica serovar Typhimurium cells rendered nonculturable by carbon and nitrogen stress in the presence of chloramphenicol were administered orally and intraperitoneally to over 300 female BALB/c mice. Neither infection nor colonization was detected in these studies, even when active but nonculturable (ABNC) cells, as defined by the Kogure cell elongation assay, were present in the inoculum. Doses of ABNC cells exceeding the oral and intraperitoneal LD50 values by 3.5 and 2 orders of magnitude, respectively, were administered. It was concluded that ABNC cells of the salmonella strains used could not be considered potentially infective and that their detection in samples from material being evaluated as a potential source or reservoir of infection by the Kogure test does not specifically represent an infective hazard.
Author(s): Barer MR; Smith RJ; Harwood CR; Newton AT
Publication type: Article
Publication status: Published
ISSN (print): 1350-0872
ISSN (electronic): 1465-2080
Publisher: Society for General Microbiology