Lookup NU author(s): Professor Jeremy Lakey
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Protein phosphorylation represents one of the major mechanisms for transcription factor activation. Here we demonstrate a molecular mechanism by which phosphorylation by mitogen-activated protein (MAP) kinases leads to changes in transcription factor activity. MAP kinases stimulate DNA binding and transcriptional activation mediated by the mammalian ETS-domain transcription factor Elk-1. Phosphorylation of the C-terminal transcriptional activation domain induces a conformational change in Elk-1, which accompanies the stimulation of DNA binding. C-terminal phosphorylation is coupled to activation Of DNA binding by the N-terminal DNA-binding domain via an additional intermediary domain. Activation of DNA binding is mediated by an allosteric mechanism involving the key phosphoacceptor residues. Together, these results provide a molecular model for how phosphorylation induces changes in Elk-1 activity.
Author(s): Lakey JH; Yang SH; Shore P; Willingham N; Sharrocks AD
Publication type: Article
Publication status: Published
Journal: EMBO Journal
Print publication date: 01/10/1999
ISSN (print): 0261-4189
ISSN (electronic): 1460-2075
Publisher: Nature Publishing Group
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