Toggle Main Menu Toggle Search

Open Access padlockePrints

The mechanism of phosphorylation-inducible activation of the ETS-domain transcription factor Elk-1

Lookup NU author(s): Professor Jeremy LakeyORCiD

Downloads

Full text for this publication is not currently held within this repository. Alternative links are provided below where available.


Abstract

Protein phosphorylation represents one of the major mechanisms for transcription factor activation. Here we demonstrate a molecular mechanism by which phosphorylation by mitogen-activated protein (MAP) kinases leads to changes in transcription factor activity. MAP kinases stimulate DNA binding and transcriptional activation mediated by the mammalian ETS-domain transcription factor Elk-1. Phosphorylation of the C-terminal transcriptional activation domain induces a conformational change in Elk-1, which accompanies the stimulation of DNA binding. C-terminal phosphorylation is coupled to activation Of DNA binding by the N-terminal DNA-binding domain via an additional intermediary domain. Activation of DNA binding is mediated by an allosteric mechanism involving the key phosphoacceptor residues. Together, these results provide a molecular model for how phosphorylation induces changes in Elk-1 activity.


Publication metadata

Author(s): Lakey JH; Yang SH; Shore P; Willingham N; Sharrocks AD

Publication type: Article

Publication status: Published

Journal: EMBO Journal

Year: 1999

Volume: 18

Issue: 20

Pages: 5666-5674

Print publication date: 01/10/1999

ISSN (print): 0261-4189

ISSN (electronic): 1460-2075

Publisher: Nature Publishing Group

URL: http://dx.doi.org/10.1093/emboj/18.20.5666

DOI: 10.1093/emboj/18.20.5666


Altmetrics

Altmetrics provided by Altmetric


Share