Lookup NU author(s): Professor Penny Lovat,
Dr Mark Dobson,
Professor Archibald Malcolm,
Professor Andrew Pearson,
Dr Chris Redfern
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Background. 9-cis retinoic acid (RA) is more effective than all-trans RA at inducing neuroblastoma differentiation in vitro, and has distinct biological properties with respect to its ability to promote apoptosis in N-type neuroblastoma cells. The cellular effects of 9-cis RA may, in part, result from activation of retinoid X receptor (RXR) homodimers. If this hypothesis is correct, 9-cis RA may control the expression of a different subset of retinoid-regulated genes compared to all-trans RA. Procedure. We have therefore used differential mRNA display to identify genes differentially expressed in neuroblastoma cells in response to all-trans and 9-cis RA. Results. The majority of cDNAs differentially expressed in response to all-trans or 9-cis RA matched to nonredundant Genbank sequences or EST database sequences. Differential-display profiles were similar in SH SY 5Y and SH S EP cells, clonal derivatives of the mixed neuroblastoma cell line SK N SH, although there were apparent differences between these cell lines with respect to the retinoid-regulation of specific RT-PCR cDNA fragments. Conclusions. These data support the view that 9-cis and all-trans RA act via different receptor mechanisms. Med. Pediatr. Oncol. 36:135-138, 2001. (C) 2001 Wiley-Liss, Inc.
Author(s): Lovat, P.E., Dobson, M., Malcolm, A.J., Pearson, A.D.J., Redfern, C.P.F.
Publication type: Article
Publication status: Unknown
Journal: Medical and Pediatric Oncology
ISSN (print): 1545-5009
ISSN (electronic): 1545-5017
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