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Evaluation of p-naphtholbenzein-beta-D-galactoside as a substrate for bacterial beta-galactosidase

Lookup NU author(s): Dr John Perry, Professor Lyle Armstrong, Professor Kate Gould

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Abstract

We describe the synthesis of a new substrate for the detection of beta -galactosidase and evaluate its performance in comparison with 5-bromo-4-chloro-3-indolyl-beta -D-galactopyranoside (X-Gal) and cyclohexenoesculetin-beta -D-galactoside (CHE-Gal). Of 206 Enterobacteriaceae strains able to hydrolyze X-Gal, 194 (94.2%) hydrolyzed CHE-Gal and 192 (93.2%) hydrolyzed p-naphtholbenzein-beta -D-galactoside (PNB-Gal). We conclude that PNB-Gal is an effective substrate for the detection of beta -galactosidase.

Publication metadata

Author(s): James AL, Chilvers KF, Perry JD, Armstrong L, Gould FK

Publication type: Article

Publication status: Published

Journal: Applied and Environmental Microbiology

Year: 2000

Volume: 66

Issue: 12

Pages: 5521-5523

ISSN (print): 0099-2240

ISSN (electronic): 1098-5336

Publisher: American Society of Microbiology

URL: http://dx.doi.org/10.1128/AEM.66.12.5521-5523.2000

DOI: 10.1128/AEM.66.12.5521-5523.2000

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