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Two-Step Assembly Dynamics of the Bacillus subtilis Divisome

Lookup NU author(s): Dr Pamela Gamba, Dr Jan-Willem Veening, Dr Leendert Hamoen, Dr Richard Daniel

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Abstract

Cell division in bacteria is carried out by about a dozen proteins which assemble at midcell and form a complex known as the divisome. To study the dynamics and temporal hierarchy of divisome assembly in Bacillus subtilis, we have examined the in vivo localization pattern of a set of division proteins fused to green fluorescent protein in germinating spores and vegetative cells. Using time series and time-lapse microscopy, we show that the FtsZ ring assembles early and concomitantly with FtsA, ZapA, and EzrA. After a time delay of at least 20% of the cell cycle, a second set of division proteins, including GpsB, FtsL, DivIB, FtsW, Pbp2B, and DivIVA, are recruited to midcell. Together, our data provide in vivo evidence for two-step assembly of the divisome. Interestingly, overproduction of FtsZ advances the temporal assembly of EzrA but not of DivIVA, suggesting that a signal different from that of FtsZ polymerization drives the assembly of late divisome proteins. Microarray analysis shows that FtsZ depletion or overexpression does not significantly alter the transcription of division genes, supporting the hypothesis that cell division in B. subtilis is mainly regulated at the posttranscriptional level.


Publication metadata

Author(s): Gamba P, Veening JW, Saunders NJ, Hamoen LW, Daniel RA

Publication type: Article

Publication status: Published

Journal: Journal of Bacteriology

Year: 2009

Volume: 191

Issue: 13

Pages: 4186-4194

ISSN (print): 0021-9193

ISSN (electronic): 1067-8832

Publisher: American Society for Microbiology

URL: http://dx.doi.org/10.1128/JB.01758-08

DOI: 10.1128/JB.01758-08


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