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The temporal and spatial expression patterns of ABCG2 in the developing human heart

Lookup NU author(s): Dr Alison Tyson-Capper, Dr Kelly Britton, Professor Stephen Robson, Dr Annette Meeson

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Abstract

Background The discovery that the adult heart is not a terminally differentiated organ and contains stem/progenitor cells has important implications for the development of cellular therapeutics to treat heart disease. Moreover the discovery of cardiac stem cells might be important in furthering our understanding of both normal and abnormal cardiac development and yet little is known about these cell populations in the developing human heart, which we have focused on in this study. Methods The presence of ABCG2 and islet-1 expressing cells in human heart was determined using immunohistochemistry and RT-PCR (and western blotting for ABCG2). Cardiac SP cells were isolated using FACS. Co-localisation immunohistochemistry was used to determine if ABCG2 positive cells expressed other known stem/progenitor cell, endothelial markers or cardiac markers. Results We observed that ABCG2 expressing cells show a difference in both their temporal and spatial patterns of expression from Islet-1 expressing cardiac progenitors. We identified rare cells that expressed both ABCG2 and markers of other cell lineages including CD31, CD34 and alpha-actinin. We also noted the presence of cells that only expressed ABCG2. We isolated cardiac SP cells and confirmed the SP cell phenotype. Conclusions Our results suggest that the developing human heart contains at least two distinct cardiac stem/progenitor cell populations one of which, the ABCG2 positive cells, can be readily isolated, suggesting that this tissue could be a useful source of cardiac stem cells.


Publication metadata

Author(s): Alfakir A, Dawe N, Eyre R, Tyson-Capper A, Britton K, Robson SC, Meeson AP

Publication type: Article

Journal: International Journal of Cardiology

Year: 2012

Volume: 156

Issue: 2

Pages: 133-138

Print publication date: 15/12/2010

ISSN (print): 0167-5273

ISSN (electronic): 1874-1754

Publisher: Elsevier Ireland Ltd

URL: http://dx.doi.org/10.1016/j.ijcard.2010.10.025

DOI: 10.1016/j.ijcard.2010.10.025


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