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B lymphocytes acquire and present intracellular antigens that have relocated to the surface of apoptotic target cells

Lookup NU author(s): Dr Marzena Ciechomska, Professor Thomas Lennard, Professor John Kirby, Dr Andrew Knight

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Abstract

The induction of an effective immune response requires the activation of CD4+ T lymphocytes by APCs. While DCs have been shown to be pivotal in this process, it is now apparent that optimal CD4+ T-cell activation also requires B-lymphocyte APC function. Along with the acquisition of soluble antigens, it is known that B cells also acquire membrane-tethered antigens. Recent reports have described the relocation of intracellular antigens to the cell surface following immunogenic apoptosis. This study was designed to determine whether B cells can acquire and present such antigens to CD4+ T cells. By targeting the model antigen tetanus toxin C fragment to various cellular locations, we show that antigen-specific B cells acquire intracellular antigens that have relocated to the surface of cells undergoing immunogenic apoptosis. Crucially, we also demonstrate that antigen-specific B cells acquiring relocated antigen from apoptotic targets are capable of efficiently inducing CD4+ T-cell activation. We propose that the acquisition and presentation of intracellular antigens that have relocated to the cell surface during immunogenic apoptosis represents a novel means by which antigen-specific B cells contribute to the generation of immunity.


Publication metadata

Author(s): Ciechomska M, Lennard TWJ, Kirby JA, Knight AM

Publication type: Article

Journal: European Journal of Immunology

Year: 2011

Volume: 41

Issue: 7

Pages: 1850-1861

Print publication date: 01/06/2011

ISSN (print): 0014-2980

ISSN (electronic): 1521-4141

Publisher: Wiley - VCH Verlag GmbH & Co. KGaA

URL: http://dx.doi.org/10.1002/eji.201141472

DOI: 10.1002/eji.201141472


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