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A comparison of a manual and an automated behavioural analysis method for assessing post-operative pain in mice

Lookup NU author(s): Dr Amy Miller, Emeritus Professor Paul FlecknellORCiD, Dr Matthew Leach, Dr Johnny RoughanORCiD

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Abstract

Current methods of identifying and analysing behavioural signs of pain in mice are highly time consuming and labour intensive. HomeCageScan (HCS) is an automated analysis system that may offer an alternative to manual scoring. Thirty-two male mice undergoing vasectomy were filmed before and at 1 h following surgery. Their behaviour was analysed by manual scoring and using an automated system (HCS; Clever Systems Inc.). The methods were compared using Bland-Altman plots, indicating a good level of agreement between the two methods for some behaviours. Other behaviours assessed showed HCS to over- or under-estimate the frequency or duration, however, when analysed using the Bland-Altman approach the two methods of analysis were still deemed interchangeable. This study has indicated that the automated analysis system may be appropriate for rapid analysis of behaviour changes in mice in some areas of biomedical research. (C) 2011 Elsevier B.V. All rights reserved.

Publication metadata

Author(s): Miller AL, Flecknell PA, Leach MC, Roughan JV

Publication type: Article

Publication status: Published

Journal: Applied Animal Behaviour Science

Year: 2011

Volume: 131

Issue: 3-4

Pages: 138-144

Print publication date: 25/03/2011

ISSN (print): 0168-1591

ISSN (electronic): 1872-9045

Publisher: Elsevier BV

URL: http://dx.doi.org/10.1016/j.applanim.2011.02.007

DOI: 10.1016/j.applanim.2011.02.007

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