Lookup NU author(s): Professor Andy Lyddiatt
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In this paper, the partition of plasmid DNA (pDNA) and RNA in polyethylene glycol (PEG) and dipotassium phosphate aqueous two-phase systems (ATPS) by adding up of NaCl salt was studied with crude Escherichia coli (E. coli) cell lysate. The partition of nucleic acid was studied in an ATPS composed of 17% (w/w) PEG300, 14% (w/w) di-potassium phosphate (designated as 17/14) and 40% (w/w) lysate. It is demonstrated that in a 17/14 system with 40% (w/w) lysate, at least 2% (w/w) or 342 mM (kg ATPS)(-1) NaCl were able to selective partition the pDNA to the bottom phase. (C) 2011 Elsevier B.V. All rights reserved.
Author(s): Luechau F, Ling TC, Lyddiatt A
Publication type: Article
Publication status: Published
Journal: Biochemical Engineering Journal
Print publication date: 05/05/2011
ISSN (print): 1369-703X
ISSN (electronic): 1873-295X
Publisher: Elsevier BV
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