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Neurogenic Properties and a Clinical Relevance of Multipotent Stem Cells Derived from Cord Blood Samples Stored in the Biobanks

Lookup NU author(s): Dr Marcin Jurga, Dr Nicolas Forraz, Christina Basford, Professor Andrew Trevelyan, Dr Saba Habibollah, Dr Simon Zwolinski, Professor Colin McGuckin

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Abstract

Several innovative therapies with human umbilical cord blood stem cells (SCs) are currently developing to treat central nervous system (CNS) diseases. It has been shown that cord blood contains multipotent lineage-negative (LinNEG) SCs capable of neuronal differentiation. Clinically useful cord blood samples are stored in different biobanks worldwide, but the content and neurogenic properties of LinNEG cells are unknown. Here we have compared 5 major methods of blood processing: Sepax, Hetastarch, plasma depletion, Prepacyte-SC, and density gradient. We showed that Sepax-processed blood units contained 10-fold higher number of LinNEG cells after cryopreservation in comparison to all other methods. We showed in this study that multipotent SCs derived from fresh and frozen cord blood samples could be efficiently induced in defined serum-free medium toward neuronal progenitors (NF200+, Ki67+). During neuronal differentiation, the multipotent SCs underwent precise sequential changes at the molecular and cellular levels: Oct4 and Sox2 downregulation and Ngn1, NeuN, and PSD95 upregulation, similar to neurogenesis process in vivo. We expect that data presented here will be valuable for clinicians, researchers, biobanks, and patients and will contribute for better efficacy of future clinical trials in regeneration of CNS.


Publication metadata

Author(s): Jurga M, Forraz N, Basford C, Atzeni G, Trevelyan AJ, Habibollah S, Ali H, Zwolinski SA, McGuckin CP

Publication type: Article

Publication status: Published

Journal: Stem Cells and Development

Year: 2012

Volume: 21

Issue: 6

Pages: 923-936

Print publication date: 06/07/2012

ISSN (print): 1547-3287

ISSN (electronic): 1557-8534

Publisher: Mary Ann Liebert, Inc. Publishers

URL: http://dx.doi.org/10.1089/scd.2011.0224

DOI: 10.1089/scd.2011.0224


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