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Lookup NU author(s): Dr Marcin Jurga, Dr Nicolas Forraz, Christina Basford, Professor Andrew Trevelyan, Dr Saba Habibollah, Dr Simon Zwolinski, Professor Colin McGuckin
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Several innovative therapies with human umbilical cord blood stem cells (SCs) are currently developing to treat central nervous system (CNS) diseases. It has been shown that cord blood contains multipotent lineage-negative (LinNEG) SCs capable of neuronal differentiation. Clinically useful cord blood samples are stored in different biobanks worldwide, but the content and neurogenic properties of LinNEG cells are unknown. Here we have compared 5 major methods of blood processing: Sepax, Hetastarch, plasma depletion, Prepacyte-SC, and density gradient. We showed that Sepax-processed blood units contained 10-fold higher number of LinNEG cells after cryopreservation in comparison to all other methods. We showed in this study that multipotent SCs derived from fresh and frozen cord blood samples could be efficiently induced in defined serum-free medium toward neuronal progenitors (NF200+, Ki67+). During neuronal differentiation, the multipotent SCs underwent precise sequential changes at the molecular and cellular levels: Oct4 and Sox2 downregulation and Ngn1, NeuN, and PSD95 upregulation, similar to neurogenesis process in vivo. We expect that data presented here will be valuable for clinicians, researchers, biobanks, and patients and will contribute for better efficacy of future clinical trials in regeneration of CNS.
Author(s): Jurga M, Forraz N, Basford C, Atzeni G, Trevelyan AJ, Habibollah S, Ali H, Zwolinski SA, McGuckin CP
Publication type: Article
Publication status: Published
Journal: Stem Cells and Development
Year: 2012
Volume: 21
Issue: 6
Pages: 923-936
Print publication date: 06/07/2012
ISSN (print): 1547-3287
ISSN (electronic): 1557-8534
Publisher: Mary Ann Liebert, Inc. Publishers
URL: http://dx.doi.org/10.1089/scd.2011.0224
DOI: 10.1089/scd.2011.0224
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