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Dermal stem cells can differentiate down an endothelial lineage

Lookup NU author(s): Dr Emma Bell, Dr Gavin Richardson, Professor Colin Jahoda, Dr Helen Phillips, Professor Deborah Henderson, Dr Andrew Owens, Dr Nicholas Hole

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Abstract

AbstractIn this study we have demonstrated that cells of neural crest origin located in the dermal papilla (DP) exhibit endothelial marker expression and functional activity. When grown in endothelial growth media, DP primary cultures up-regulate expression of FLT1 (VEGF receptor) and down-regulate expression of the dermal stem cell marker alpha-smooth muscle actin. DP cells have demonstrated functional characteristics of endothelial cells: including the ability to form capillary-like structures on Matrigel, increase uptake of low density lipoprotein and up-regulate ICAM1 in response to TNFalpha stimulation. We confirmed that these observations were not due to contaminating endothelial cells, by using DP clones. We have also used the WNT1cre/ROSA26R and WNT1cre/YFP lineage tracing mouse models to identify a population of neural crest derived cells in DP cultures that express the endothelial marker PECAM; these cells also form capillary-like structures on Matrigel. Importantly, cells of neural crest origin that express markers of endothelial and mesenchymal lineages exist within the dermal sheath (DS) of the vibrissae follicle.


Publication metadata

Author(s): Bell E, Richardson G, Jahoda CA, Gledhill K, Phillips HM, Henderson D, Owens WA, Hole N

Publication type: Article

Journal: Stem Cells and Development

Year: 2012

Volume: 21

Issue: 16

Pages: 3019-3030

Print publication date: 09/05/2012

ISSN (print): 1547-3287

ISSN (electronic): 1557-8534

Publisher: Mary Ann Liebert, Inc. Publishers

URL: http://dx.doi.org/10.1089/scd.2011.0694

DOI: 10.1089/scd.2011.0694


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