Toggle Main Menu Toggle Search

ePrints

Enzyme activity demonstrates multiple pathways of innate immunity in Indo-Pacific anthozoans

Lookup NU author(s): Caroline Palmer, Professor John Bythell

Downloads

Full text for this publication is not currently held within this repository. Alternative links are provided below where available.


Abstract

Coral reefs are threatened by increasing levels of coral disease and the functional loss of obligate algal symbionts (bleaching). Levels of immunity relate directly to susceptibility to these threats; however, our understanding of fundamental aspects of coral immunology is lacking. We show that three melanin-synthesis pathway components (mono-phenoloxidase, ortho-diphenoloxidase (tyrosinase-type pathway) and para-diphenoloxidase (laccase-type pathway)) are present in both their active (phenoloxidase, PO) and inactive (prophenoloxidase, PPO) forms across a diverse range of 22 species of healthy Indo-Pacific anthozoans. We also demonstrate transglutaminase activity of the coagulation cascade for, to our knowledge, the first time in a coral. Melanin-synthesis enzyme activities varied among taxa, although they were generally lowest in the coral family Acroporidae and highest in the Poritidae and Oculinidae. Inactive tyrosinase-type activity (PPO) and active laccase-type activity (PO) correlate with taxonomic patterns in disease resistance, whereas the converse pattern in activity levels correlates with bleaching resistance. Overall, we demonstrate the presence of several melanin-synthesis pathways in Indo-Pacific corals, co-regulation among some pathway components, and highlight their potential roles in coral health.


Publication metadata

Author(s): Palmer CV, Bythell JC, Willis BL

Publication type: Article

Journal: Proceedings of the Royal Society B: Biological Sciences

Year: 2012

Volume: 279

Issue: 1743

Pages: 3879-3887

Print publication date: 22/09/2012

ISSN (print): 0962-8452

ISSN (electronic): 1471-2954

Publisher: The Royal Society Publishing

URL: http://dx.doi.org/10.1098/rspb.2011.2487

DOI: 10.1098/rspb.2011.2487


Altmetrics

Altmetrics provided by Altmetric


Actions

    Link to this publication


Share