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Impact of collagen/heparin multi-layers for regulating bone cellular functions

Lookup NU author(s): Dr Ana Ferreira-Duarte, Dr Piergiorgio Gentile, Dr Ria Toumpaniari, Dr Mark Birch



Bone cell interaction with extracellular matrix (ECM) microenvironment is of critical importance when engineering surface interfaces for bone regeneration. In this work layer-by-layer films of type I collagen (coll), the major constituent of bone ECM, and heparin (hep), a glycosaminoglycan, were assembled on poly(L-lactide acid) (PLLA) substrates to evaluate the impact of the biomacromolecular coating on cell activity. The surface modification of PLLA demonstrated that the hep/coll multi-layer is stable after 10 bilayers (confirmed by contact angle, infrared spectroscopy and morphological analysis). This simple approach provided novel information on the effect of heparin on type I collagen hierarchical organisation and subsequent cell response of osteoblast-like (MC3T3-E1) and human bone marrow-derived mesenchymal stem cells (hMSCs). Interestingly, the number of deposited heparin layers (1 or 10) appeared to play an important role in the self-assembly of collagen into fibrils, stabilising the fibrous collagen layer, and potentially impacting on hMSCs activity.

Publication metadata

Author(s): Ferreira AM, Gentile P, Toumpaniari S, Ciardelli G, Birch MA

Publication type: Article

Journal: ACS Applied Materials & Interfaces

Year: 2016

Volume: 8

Issue: 44

Pages: 29923-29932

Online publication date: 20/10/2016

Acceptance date: 18/10/2016

ISSN (print): 1944-8244

ISSN (electronic): 1944-8252

Publisher: American Chemical Society


DOI: 10.1021/acsami.6b09241


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