Toggle Main Menu Toggle Search

ePrints

Biosynthetic PCL-graft-collagen bulk material for tissue engineering

Lookup NU author(s): Dr Piergiorgio Gentile, Kegan McColgan-Bannon, Professor Kenneth Dalgarno, Dr Ana Ferreira-Duarte

Downloads

Full text for this publication is not currently held within this repository. Alternative links are provided below where available.


Abstract

Biosynthetic materials have emerged as one of the most exciting and productive fields in polymer chemistry due to their widespread adoption and potential applications in tissue engineering (TE) research. In this work, we report the synthesis of a poly(ε-caprolactone)-graft-collagen (PCL-g-Coll) copolymer. We combine its good mechanical and biodegradable PCL properties with the great biological properties of type I collagen as a functional material for TE. PCL, previously dissolved in dimethylformamide/dichloromethane mixture, and reacted with collagen using carbodiimide coupling chemistry. The synthesised material was characterised physically, chemically and biologically, using pure PCL and PCL/Coll blend samples as control. Infrared spectroscopy evidenced the presence of amide I and II peaks for the conjugated material. Similarly, XPS evidenced the presence of C–N and N–C=O bonds (8.96 ± 2.02% and 8.52 ± 0.63%; respectively) for PCL-g-Coll. Static contact angles showed a slight decrease in the conjugated sample. However, good biocompatibility and metabolic activity was obtained on PCL-g-Coll films compared to PCL and blend controls. After 3 days of culture, fibroblasts exhibited a spindle-like morphology, spreading homogeneously along the PCL-g-Coll film surface. We have engineered a functional biosynthetic polymer that can be processed by electrospinning.


Publication metadata

Author(s): Gentile P, McColgan-Bannon K, Ceretto N, Sefat F, Dalgarno K, Ferreira AM

Publication type: Article

Journal: Materials

Year: 2017

Volume: 10

Issue: 7

Pages: 693-710

Online publication date: 23/06/2017

Acceptance date: 19/06/2017

ISSN (electronic): 1996-1944

Publisher: MDPI

URL: http://www.mdpi.com/1996-1944/10/7/693

DOI: 10.3390/ma10070693


Altmetrics

Altmetrics provided by Altmetric


Actions

    Link to this publication


Share