Lookup NU author(s): Dr Ajay Kohli
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We discuss mechanisms and factors that influence levels and stability of expressed heterologous proteins in crop plants. We have seen substantial progress in this field over the past two decades in model experimental organisms such as Arabidopsis and tobacco. There is no question such studies have resulted in furthering our understanding of key processes in the plant cell and the elaboration of sophisticated models to explain underlying mechanisms that might influence the fate, levels and stability of expression of recombinant heterologous proteins in plants. However, very often, such information is not applicable outside these laboratory experimental models. In order to generate a knowledge basis that can be used to achieve high levels and stability of heterologous proteins in relevant crop plants it is imperative to perform such studies on the target crops. With this in mind, we discuss key elements of the process at the DNA, RNA and protein levels. We believe it is essential to discuss recombinant protein production in crops in a holistic manner in order to develop a comprehensive knowledge base that will in turn serve plant biotechnology applications well.
Author(s): Kohli A, Melendi PG, Abranches R, Capell T, Stoger E, Christou P
Publication type: Review
Journal: Plant Signalling & Behavior
ISSN (print): 1559-2316
ISSN (electronic): 1559-2324
Notes: This review is unique in that for the first time it brings together the transgenic DNA as well as the transgenic protein related reasons for transgene expression and silencing. It provides and overview on how best to obtain the required amounts of the transgenic products and what factors to consider at the DNA, RNA and the protein level to achieve that.