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Lookup NU author(s): Dr Michail Karavolos, Dr David Bulmer, Dr Jeong-Jin Lee, Dr Anjam Khan
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Pathogenic bacteria employ a variety of mechanisms to resist a barrage of stresses they encounter during active growth in or outside the host as well as during growth stasis. An in silico screen of the Salmonella genome sequence revealed that Salmonella typhimurium LT2 possesses a homologue belonging to the universal stress protein A (UspA) family. We assessed the transcriptional profile of uspA in S. typhimurium C5 by constructing a lacZ fusion revealing that uspA is induced by metabolic, oxidative, and temperature stresses. The highest transcriptional levels occurred in cells entering stationary phase, an observation consistent with expression patterns in Escherichia coli. The protein was purified as a fusion with GST (UspAF) and antibodies raised against UspAF revealed elevated protein levels in stressed and growth-arrested cells. Inactivation of uspA in S. typhimurium C5, lead to increased susceptibility to stress conditions. Furthermore, UspA makes an important contribution to the in vivo virulence of Salmonella in mice thus highlighting the importance of stress resistance regulation in pathogenicity and survival within the host. Crown Copyright © 2006.
Author(s): Liu W-T, Karavolos MH, Bulmer DM, Allaoui A, Hormaeche RDCE, Lee JJ, Anjam Khan CM
Publication type: Article
Publication status: Published
Journal: Microbial Pathogenesis
Year: 2007
Volume: 42
Issue: 1
Pages: 2-10
ISSN (print): 0882-4010
ISSN (electronic): 1096-1208
Publisher: Academic Press
URL: http://dx.doi.org/10.1016/j.micpath.2006.09.002
DOI: 10.1016/j.micpath.2006.09.002
PubMed id: 17081727
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