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Lookup NU author(s): Dr Emma Waite
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The proportion of D- to L-enantiomers of aspartic acid in metabolically isolated proteins has been used by forensic scientists to estimate ape at death. We have demonstrated the interference of a derivative of hydroxyproline (N-TFA isopropyl Hyp ester) with the N-TFA isopropyl L-Aspartic (Asp) acid ester during gas chromatography of amino acids. This has serious implications for the accurate quantification of the D- to L-Asp ratio extracted from collagenous proteins. Having demonstrated the potential for this co-elution in amino acid standards, acid-soluble dentine proteins and non-mineralised collagen, we argue that this problem can be overcome either by high resolution separation or by analysis of the (Hyp-poor) non-collagenous protein fraction.
Author(s): Waite ER, Collins MJ, van Duin ACT
Publication type: Article
Publication status: Published
Journal: international Journal of Legal Medicine
Year: 1999
Volume: 112
Issue: 2
Pages: 124-131
Print publication date: 01/01/1999
ISSN (print): 0937-9827
ISSN (electronic): 1437-1596
Publisher: Springer
URL: http://dx.doi.org/10.1007/s004140050214
DOI: 10.1007/s004140050214
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